Amino Acid Column Hplc

In an amino column you have an nh2 group at the end of the carbon chain attached to the silica.

Amino acid column hplc.

The nh2 hplc column uses the innovative filler bonding technology unique to hawach. Lc ms analysis of amino acids without derivatization. The amino acid hplc column is a dedicated column developed by hawach for amino acid analysis. Amino acid analysis on eclipse plus c18 2 1 3 0 or 4 6 x 50mm 1 8µ column.

Optimized stationary phase for the separation of amino acids and other amine containing compounds. Hydrolysed samples amino acids are derivatized for sensitive detection separated by hplc. It bonds with aminopropyl functional groups and through strict process control to ensure the formation of even dense amino functional groups. We are planning to develop method on analysis of amino acids in food matrix cereals pulses oil seeds etc by hplc with pre or post column derivatization methods since this is new to us so could.

Pre column derivatizing reagents most typically used for amino acid analysis are o phthalaldehyde phenyl isothiocyanate pitc fluorescamine and dansyl chloride. The characteristics of the column will be affected by the chemical properties of this functional group. Experimental conditions aaa on production 1200sl using eclipse plus c18 2 1 or 4 6 x 50mm 1 8u. However pre column derivatization has gained importance and can be achieved by a broader range of derivatization reagents.

Description of amino acid hplc columns. Since most of the amino acids have no absorption in the ultraviolet. Post column derivatization was typical. Nh2 hplc columns description.

Full chromatographic resolution of isobaric compounds like leu and ile. Therefore pre column derivatization can be considered appropriate when intending to analyze a somewhat limited variety of samples with high sensitivity. The use of internal and external standards is crucial. Whether you are looking to separate proteins peptides or amino acids the intrada series of columns has a solution for you.

It uses ultra high purity fully porous spherical silica as a matrix to provide a good peak for amino acid analysis using a unique bonding process and a more thorough tailing process.